Spider silks could become the intelligent materials of the future, according to a review article published this month in the journal Microbial Cell Factories. The characteristics of spider silk could have applications in areas ranging from medicine to ballistics.

The distinctive toughness of spider silk could allow manufacturers to improve wound-closure systems and plasters, and to produce artificial ligaments and tendons for durable surgical implants. The silk could also be woven into strong textiles to make parachutes, body armour, ropes and fishing nets. A whole range of ecological materials could emerge from the industrial production of spider silk.

Thomas Scheibel, from the Department of Chemistry of the Technische Universität in München explains that there are currently over 34,000 described species of spider, each with a specific tool-kit of silks with different mechanical properties serving specific purposes.

For example, major ampullate silk, a very tough silk with a tensile strength comparable to Kevlar, is used for the primary dragline or scaffolding of the spider’s web. Minor ampullate silk with its very low elasticity is used to reinforce the web, while the strong and stretchy flagelliform silk forms the capture spiral of the web.

Biotechnologists are currently analysing the properties of silk proteins and how they assemble into threads. Knowing exactly how silk fibers are formed and what mechanical properties result from different assembly processes could allow the manufacture of artificial spider silks with special characteristics such as great strength or biochemical activity.

“The future objective might not be to prepare identical copies of natural silk fibers, but rather to capture key structural and functional features in designs that could be useful for engineering applications” explains the author.

Spiders are territorial and cannibalistic and so impossible to farm. The only way to produce large quantities of silk is to engineer and insert silk genes into other cells or organisms. But this has been complicated by the nature of the genes, which include many repeated sequences and rely on a different codon reading system from ours. However, in recent studies parts of the genes were successfully inserted into the bacterium E. coli, mammal and insect cells, which in turn produced silk proteins.

“Using ‘protein engineering’ based on knowledge achieved from investigations of the natural silks, artificial proteins can be designed that allow bacterial synthesis at high yields” writes Scheibel in the article*.

Engineering new proteins would also allow the design of completely new types of silk fiber, which could assemble with biochemically or biologically active groups into new types of mesh. These ‘intelligent’ materials would then be able to carry out enzymatic reactions, chemical catalysis or electronic signal propagation, for example.

Before this can be achieved, the spinning of proteins into fibers has to be resolved. So far there have been a few attempts at spinning silk on silicon micro-spinnerets. The outcomes have been promising but are far from matching naturally produced silks. For the moment the fibers produced are too wide, with diameters ranging from 10 to 60mm, compared with diameters of 2.5 to 4.0mm in natural fibers.

Notes:
This press release is based on the following article:

Spider silks: recombinant synthesis, assembly, spinning, and engineering of synthetic proteins Thomas Scheibel
Microbial Cell Factories 2004, 3:14 (16 November 2004)

For further information about this study, contact Dr. Thomas Scheibel by email at thomas.scheibel@ch.tum.de or by phone at +49 179 127 5296

Alternatively, or for more information about the journal, contact Juliette Savin by e-mail at press@biomedcentral.com or by phone on +44 (0)20 7631 9931

Microbial Cell Factories (http://www.microbialcellfactories.com/home/) publishes original research on all aspects of the development, use and investigation of microbial cells as factories for production of both natural products and recombinant proteins.
Microbial Cell Factories is published by BioMed Central (http://www.biomedcentral.com), an independent online publishing house committed to providing Open Access to peer-reviewed biological and medical research. This commitment is based on the view that immediate free access to research and the ability to freely archive and reuse published information is essential to the rapid and efficient communication of science. BioMed Central currently publishes over 100 journals across biology and medicine. In addition to open-access original research, BioMed Central also publishes reviews, commentaries and other non-original-research content. Depending on the policies of the individual journal, this content may be open access or provided only to subscribers.



Peer reviewed publication and references

Spider silks: recombinant synthesis, assembly, spinning, and engineering of
synthetic proteins
Thomas Scheibel
Microbial Cell Factories 2004, 3:14 (16 November 2004)
http://www.microbialcellfactories.com/content/3/1/14